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Protein Purification and Crystallization of Diabetes Related Peroxisome Proliferator-Activated Receptor-ɣ (PPARɣ)

PPARɣ plays a role in glucose metabolism. Activated PPARɣ increases insulin sensitivity, making PPARɣ an important receptor for treatment of type 2 diabetes. Obtaining a crystal structure of PPARɣ aids in development of interacting ligands and beneficial anti-diabetic drugs. Here we describe the purification of PPARɣ using Bio-Rad's NGC™ chromatography system and a three-step column purification approach. Well-ordered crystals of the purified PPARɣ protein were obtained. X-ray diffraction data showed consistent results compared to established protocols. 

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