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Workflow for Protein Purification from Whey Using Nuvia™ Ion Exchange Media 

Whey proteins play a vital role in the formulation of food supplements because of numerous desirable nutritional and functional properties. Here we present a process for whey purification using Nuvia S and Nuvia Q ion exchange media. The major whey proteins are β-lactalbumin (ALA), 13-lactoglobulin (BLG), bovine serum albumin (BSA), and bovine immunoglobulins (Hahn et al. 1998) (Table 1). Minor proteins such as lactoperoxidase, lactoferrin, and proteose-peptone account for the other protein components in whey. 

The objective of this study was to develop a separation process to fractionate usable proteins from whey (Figure 1). First, we purified a total whey protein isolate (WPI) because of its high-end functional advantages, as evidenced by a demand from nutrition and health segments. Secondly, ALA and BLG were purified because of their value in infant formula and confections, respectively. 

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