Bio-Rad Sponsored Content

Monoclonal Antibody Purification Using UNOsphere SUPrA™ Media 

The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies (Abs), is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. They bind with high affinity to the , region of most subclasses of antibodies and are one of the standard tools used in antibody capture and purification. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed mAb drugs. 

UNOsphere SUPrA affinity chromatography medium is a protein A-based resin developed by Bio-Rad Laboratories, Inc., based on the proven UNOsphere™ matrix. UNOsphere SUPrA media can be integrated in a workflow of mAb purification in combination with UNOsphere Q media and CHT™ ceramic hydroxyapatite, as described in this tech note. The results demonstrate that UNOsphere SUPrA media in combination with the subsequent polishing steps delivers highly pure mAbl and mAb2. 

View full application note (PDF)