The first step in purification of an important class of therapeutic proteins, the polyclonal or monoclonal antibodies (Abs), is their capture from plasma or tissue culture supernatants. Protein A-based media are by far the most common class of affinity products used for this purpose. They bind with high affinity to the , region of most subclasses of antibodies and are one of the standard tools used in antibody capture and purification. In combination with ion exchange and ceramic hydroxyapatite chromatography, protein A-based media have been successfully used in the large-scale purification of numerous licensed mAb drugs.
UNOsphere SUPrA affinity chromatography medium is a protein A-based resin developed by Bio-Rad Laboratories, Inc., based on the proven UNOsphere™ matrix. UNOsphere SUPrA media can be integrated in a workflow of mAb purification in combination with UNOsphere Q media and CHT™ ceramic hydroxyapatite, as described in this tech note. The results demonstrate that UNOsphere SUPrA media in combination with the subsequent polishing steps delivers highly pure mAbl and mAb2.