Bio-Rad Sponsored Content

Plasmid DNA Adsorption Behavior on CHT™ Ceramic Hydroxyapatite Type II Support

Plasmid DNA adsorbs to the surface of most commercially available chromatographic supports. These chromatographic supports were designed and developed for protein purification and thus have relatively small pore dimensions. The small pores lead to low adsorption capacity of plasmid DNA on the support; in fact, binding capacities for plasmid DNA average 50 times less than those for protein (Ferreira et al. 2000). There is thus a need to optimize the adsorption capacity for plasmid DNA on most of the chromatographic supports currently available. Previously we demonstrated that chromatography on CHT ceramic hydroxyapatite Type II yielded plasmid of near pharmaceutical grade in a single step (Aberin and Franklin 2002). Here we report on the effect of size and type of plasmid DNA, as well as the nature of the buffer system, on the dynamic binding capacity (DBC) and recovery on CHT Type II support. 

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